PHARMACODYNAMIC INTERACTION OF TINOSPORA CORDIFOLIA WITH ATENOLOL AND PROPRANOLOL IN ISOPROTERENOL INDUCED CARDIAC NECROSIS IN RATS

ABSTRACT Objective: In the present scenario high rate of mortality is mainly because of cardiovascular diseases globally.  Hence, there is need for extensive research for effective treatment of these cardiovascular diseases. The present study was carried out with aim to evaluate the combined cardioprotective effect of standardized extract of Tinospora cordifolia extract (TCE) with atenolol (AT) and propranolol (PP) in isoproterenol (ISO) induced cardiac necrosis in rats. Methods: Myocardial infarction (MI) or cardiac necrosis was induced by subcutaneous administration of ISO for two days consecutively at an interval of 24 h. Rats were pre-administered with test drugs for  21 days followed by ISO was administration on 20 and 21st day. 24 h after final ISO administration mean arterial blood pressure (MAB), Heart rate (HR), electrocardiogram (ECG), heart bio-marker enzyme and histopathological study of cardiac tissue were evaluated from control and experimental groups and analyzed statistically by one-way ANOVA followed by Tukeys’s test. Results: Rats administered with ISO showed significant changes in ECG, HR, MAB, heart bio-marker enzyme, antioxidant parameters and histopathology of heart. The activities of biomarkers have reduced in serum and there is increase antioxidants in heart tissue of drug treated animals. Similarly, ECG, MAB and HR were restored to normalcy in drug treated animals. Conclusion: It may be concluded that the herb-drug combinations i.e TCE (500 mg/kg) + AT (10 mg/kg) and TCE (500 mg/kg) + PP (10 mg/kg) have shown increased cardioprotective activity than they were used alone.