Effects of plant transfer ribonucleic acids on interferon production.

: An optimal interferon (IFN) production was obtained at concentrations of 50 micrograms/ml poly I:C and 2000 micrograms/ml DEAE dextran in Lpa cells. It was shown that methionine initiator tRNA (tRNAiMet) in a dose 50 micrograms/ml or crude tRNA (tRNAc) applied to Lpa cell during the stages of IFN induction, IFN induction and synthesis, as well as during IFN synthesis resulted in a continuous IFN production for up to 24 hr. Exposure of the cells to 150 micrograms/ml tRNAiMet during the stages of IFN induction and IFN induction and synthesis caused total inhibition of IFN production. This effect was partially observed only after the high dose of tRNAc. Addition of a high dose of tRNAiMet or tRNAc to cells during the synthesis stage caused no inhibition but prolongation of IFN production.