Effect of Sodium Ferulate on Myocardial Ischemia Fibrosis Induced by Isoproterenol

We examined whether the powerful sodium ferulate(SF) could improve myocardial ischemia fibrosis de- gree and gain the information of myocardial energy metabolism via experimental model of myocardial ischemia fi- brosis. The model of myocardial ischemia fibrosis was made for the wistar rats induced with 15 mg/kg isoprotere- nol(Iso) subcutaneous injection. In experiment 1, Iso effective model drug of myocardial ischemia fibrosis at present was administrated to the rats with myocardial ischemia fibrosis and 2, 4, 6, 12, 24, 48 and 72 h, 7 and 21 d later, the changes of adenosine content in rat myocardial tissue and hydroxyproline in blood serum were compared. In experi- ment 2, SF was administrated to the rats with myocardial ischemia fibrosis and 21 d later, the effect of SF on benaze- pril as reference drug curing myocardial ischemia fibrosis was evaluated by measuring the changes of adenosine in rat myocardial tissue and hydroxyproline content in blood serum. In the model group, there was a remarkable increase in hydroxyproline content and a decrease in adenosine content of myocardial tissue in experiment 1; there was a re- markable decrease in hydroxyproline in blood serum and a increase in adenosine content in myocardial tissue, which were recovered gradually to control in experiment 2(P<0.05). The results of experiment 1 show that with the increa- sing of pathological change degree, the rat adenosine content reduced gradually and arrived at the lowest value three weeks later(P<0.05); the hydroxyproline content was obviously higher compared with that of control group(P<0.05). The results of experiment 2 show that rat adenosine content in myocardial tissue and hydroxyproline content in blood serum were recovered gradually to normal level after injection SF. The effect of SF against myocardial ischemia fi- brosis in preventive group is better than that of improving the myocardial ischemia fibrosis drug. Meanwhile, hinting HPLC is a good method to measure the adenosine content.