Development and Validation of a UV-Spectrophotometric Method for Quantitative Determination of Benzene Fragments in Benzyl Esters of Heparin

A new UV-spectrophotometric method for determining the concentration of benzyl fragments in heparin benzyl esters is proposed. The method was validated for linearity, accuracy, and precision in terms of repeatability and intralaboratory reproducibility. The method is intended to characterize heparin benzyl esters, which are intermediates in the synthesis of low-molecular-weight heparin via hydrolytic depolymerization of high-molecular-weight heparin.