Study on the Genetic Transformation of Gentian by Gene Recombinant

Transformation recombinant vector pMHL7133-Gus linked with rol gene which be cloned from Agrobacterium Rhizogenes R1000 through Agrobacterium tumefaciens LBA4404 into explant of gentian lamina, inducing rol gene express and producing hair root. Meanwhile, using the Agrobacterium Rhizogenes R1000 infect gentian directly as a comparison, we built two sets of transform system of Agrobacterium for hairy root through researching on all kinds of factors deeply and optimizing transform condition. As a result, the production rate of induced hairy root using Ti plasmid to mediated recombinant vector transforming Gentian is better than the hairy root mediated by Ri plasmid. (Nature and Science. 2006;4(4):60-67).