Storage Stability of pCTFE Carboxylic Acid Metabolites.

Abstract : Studies conducted at the Toxic Hazard Research Unit have analyzed trimer and tetramer carboxylic acid metabolites of polychlorotrifluoroethylene (pCTFE) in biological samples. Tissue samples have been stored at -70 deg C, and urine and feces have been stored at -20 deg C for periods of time ranging from several months to over 1 year. At the time of these analyses, the storage stability of the carboxylic acid metabolites of pCTFE was not known. To investigate the storage stability of the carboxylic acid metabolites of pCTFE, male Fischer 344 rats were dosed orally with pCTFE parent compound and sacrificed after 48 h. Samples of urine were collected from 24 to 48 h after dosing, and liver, kidney, lung, and blood were collected at 48 h. Samples were analyzed via gas chromatography with electron capture detection. Concentrations of the carboxylic acid metabolites were measured at 2 weeks, 6 weeks, 17 weeks, and 55 weeks. The stability data were analyzed using a 1-factorial multivariate analysis of variance with trend analysis to evaluate the storage stability of the pCTFE carboxylic acid metabolites.