Real-time quantification of microRNA by poly(U)-tailed RT-PCR

Objective To establish a new method of poly(U)-tailed RT-PCR to detect microRNA(miRNA).Methods The total RNA of cells was extracted and polyuridylylated by poly(U) polymerase,then the cDNA was obtained by reverse transcriptase with the universal primer,and the miRNA was detected by real-time quantitative PCR.Results This method of poly(U)-tailed RT-PCR could better detect miRNA with specificity and efficiency.Conclusion This method of poly(U)-tailed RT-PCR for detection of miRNAs is simple,economical and efficient.