Measurement of nucleotide excision repair activity and correlation with cisplatin sensitivity.

1077 Background: Nucleotide excision repair (NER) of DNA is necessary for removal of crosslinks and other damage produced by UV light, genotoxins and chemotherapies. Germline NER deficiency causes Xeroderma Pigmentosum (XP) and inevitably leads to cancer. We explored the relationships between NER, breast cancer (BC), and cisplatin, a platinum-based chemotherapy. NER removes platinum-induced DNA crosslinks. Ergo, NER deficits may contribute to sensitivity to cisplatin-based chemotherapies. Methods: We assessed NER deficiency and sensitivity to cisplatin and UV in 40+ BC cell lines, including various subtypes as presented by published sources (COSMIC database from Forbes et al, 2014, and Neve et al Cancer Subtype Study, 2006). To monitor NER we measured removal of [6-4] photoproducts from UV-irradiated cells using a DDB2 proteo-probe (Dreze et al. 2014). Data analysis was based in Prism with t-tests and correlation studies. Results: The vast majority of tested cell lines, both cancerous and non-cancerous, e...