Experiment study on enhancement of antitumor effect of mitomycin C by verapamil in human Cervical Cancer

Objective To determine the reversion of mitomycin(MMC)resistance by verapamil (VER)in vitro and in vivo.Methods A xenografted mitomycin resistant mice model of cervical cancer was devoleped.The reversion of mitomycin of resistance by VER was detected for human cervical cancar cell(hela)and its resistant subline Hela/MMC in vitro and in vivo.Results nontoxin doses of VER might enhance the cytotoxicity of MMC in Hela and Hela/MMC,could result in almost complete or partial reversion of MMC resisistance of Heia/MMC, 3H TdR incorporation test indicated that VER enhanced the inhibition of DNA synthesis in Hela and Hela/MMC cell. Conclusion VER can overcome mitomycin resistance of Hela/MMC in vitro and in vivo.