An FTIR study of Pseudomonas aeruginosa PAO1 biofilm growth and dispersion. An improved ATR method for studying biofilms: the C–H stretch spectral region

Pseudomonas aeruginosa PAO1 was grown on 40° ZnSe and 40° AMTIR® ( A morphous M aterial T ransmitting I nfrared R adiation, Ge 33 As 12 Se 55 ) internal reflection elements in a thermostatted flow cell. Information about the chemistry of the biofilm development was obtained using Fourier transform infrared spectroscopy. The weakly absorbing C–H stretching region of the infrared spectrum was measured for the first time and revealed qualitative and quantitative changes not evident in the amide region studied by previous investigators. There is a chemical difference between the early growth period and subsequent development of the biofilm.