Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice

Objective To observe the effect of cervical sympathetic block(SB) on NF-κB and its coactivator during the early stage in burned mice.Methods Kunming male mice were randomly divided into normal control,burn control,LSB group(treated immediately by SB with Lidocaine after burn injury) and RSB group(treated immediately by SB with Ropivacaine after burn injury).Third degree burn injury involving 15%-20% of TBSA was produced.At 6 h after burn,the expression and nuclear translocation of hepatic NF-κB P65 and steroid receptor coactivator-3(SRC-3) were detected by Western blotting.Results After burn injury,the expression and nuclear translocation of hepatic NF-κB P65 and SRC-3 were markedly elevated as compared with normal control group.The level of SRC-3 and nuclear translocation of NF-κB P65 and SRC-3 in LSB group were significantly downregulated compared with that of burn control group,but the NF-κB P65 was no significant difference with that of burn control group.The expression and nuclear translocation of NF-κB P65 and SRC-3 in RSB group were obviously lower than burn control group and LSB group.Conclusion The expression and nuclear translocation of hepatic NF-κB P65 and SRC-3 are markedly elevated during the early stage of burn injury.SB can exert supression on the function of NF-κB and reduce the degree of systemic inflammatory response,which may be the mechanisms for therapeutic effect of SB on severe trauma.