Antibacterial aromatic compound and steroid glycoside isolated from duckweed Lemna paucicostata Hegelm

Lemna paucicostata Hegelm is traditionally used as healthy feed for fish, broilers and as a source for vitamin A. In the present study, we report the isolation of an aromatic compound and a steroid glycoside from Lemna pauciscostata. The methanolic extract of dried and powdered Lemna paucicostata was fractionated using solid phase extraction (SPE). The compound was collected in a series of fractions. While first fraction contributed (80% pure) to an aromatic compound, 3 rd fraction contained a steroid glycoside. Preliminary analysis of the compounds was performed with nuclear magnetic resonance and mass spectrometry. Aromatic compound was analyzed by 1 H NMR and MS spectra, steroid glycoside were analyzed by 1 H NMR and IR spectra. Aromatic compound showed significant inhibition of growth (10 mm) against Gram-negative bacteria, Pseudomonas aeruginosa.