Frequencies of SDF-1 chemokine, CCR-5, and CCR-2 chemokine receptor gene alleles conferring resistance to human immunodeficiency virus type 1 and AIDS in Kuwaitis

The frequencies of three mutations conferring resistance to HIV/AIDS were determined in a population sample of native Kuwaitis. The CCR2-64I, SDF1-3′A, and CCR5-m303 mutations were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) tests using restriction endonucleases Bsa BI, Msp I, and Hinc II, respectively. The frequency of the mutant alleles were: for CCR2-64I, 0.1195 (95% CI 0.0801–0.1694); for SDF1-3′A, 0.2593 (95% CI 0.2024–0.3231), and for CCR5-m303, less than 0.0025. Thus, the CCR2-64I and especially SDF1-3′A mutations are sufficiently common in Arabs and can be used for prognostic genotyping in HIV-infected individuals from the Gulf countries. J. Med. Virol. 58:54–58, 1999. © 1999 Wiley-Liss, Inc.