The effect of daucosterol in achyranthes bidentata bl. on proliferation of umr106 osteoblast like cells and itsdetermination

Daucosterol isolated from AB was cultured with UMR106 OB-like cells together in vitro and the proliferatioa of OB-like cells determined by MTT assay. The chromatographi condition dtermining daucosterol include an ODS column (250mm*4.6mm,5#mu#m), a mobil phase consisting of a ixture of water-acetontrile (70:30), detection wavelength of 203nm,and a column temperature of 30deg,Phenacetin was used as the interal standard. Results:The daucostero from AB had significant effect on Phenacetion of OB-like cells, the proliferation was promoted by 45.8%(n=3). The average recovery of daucosterol was 94.2% (RSD=3.2%),the calibration was linear in the range of 4-40#mu#g/mL (r=0.9988). Daucosterol was screened quickly by cultivating with OB-like cells together in vitro The HPLC method is acurate, fast and repeatable for the determination of daucosterol in AB.