Differential disc electrophoresis of serum proteins.

SummaryA polyacrylamide electro-phoretic procedure, in which a complex ionic mixture migrates, through both a large pore and a small pore size gel on a single column is described. The procedure is simple, flexible and results in better separation and resolution of serum proteins. Human serum separated into 44-50 components; and bovine serum into over 30 bands. An economical apparatus for the rapid staining and destaining of disc gels is described which eliminates handling of the gels during the staining and destaining processes.