Study on toxicity of hyperoside in rat embryo-fetal development

Objective: To observe the toxicity of hyperoside in rat embryo-fetal development,in order to provide preference for safe use of drugs during gestation period.Method: Healthy pregnant rats were randomly divided into hyperosid groups(30,175,1 000 mg·kg-1·d-1),the positive control group(cyclophosphamide,7 mg·kg-1·d-1) and the solvent control group(1% aqueous carboxymethylcellulose).These rats were orally administered with hyperosid or vehicle during 6-15 d after gestation and subcutaneously injected with cyclophosphamide during 11-13 d.Maternal clinical sign,abortions,premature deliveries and body weight were monitored throughout gestation.At termination(gestation days 20),pregnant females were evaluated for clinical symposiums,weight change,corpora lutea count,existence and death of embryos;live fetuses were examined for gender,external,visceral and skeletal malformation and variations.Result: All pregnant rats showed no significant abnormality in appearance,viscera and skeletal development.However,there was a difference between the high-dose group of hyperoside and negative control group in the fetus body weight,the length of the embryos and the length of tail(P 0.05).Conclusion: Pregnant women are suggested to cautiously use hyperoside because it shows certain impact on development of fetal rats under the experimental conditions.