Relations between the new long non-coding RNA UC001kfo and the metastasis of liver cancer

Objective Investigate the expression of a new long non-coding RNA (IncRNA) UC001kfo in liver cancer and its relations to the invasion and metastasis of liver cancer.Methods Collect 10 cases of liver cirrhosis tissue samples,25 cases of liver cancer tissue samples,25 cases of samples of the tissues near the cancer and 10 cases of samples of the tissues of portal vein transferred liver cancer.The in-situ hybridization method is used to test the expression of UC001kfo and ACTA2 ( gene of α-smooth muscle actin) in liver cancer tissues.Results Results of the in-situ hybridization indicate that UC001kfo is not expressed or expressed in low quantity in the liver cirrhosis group,but it is expressed positively in all the liver cancer tissues,tissues near the cancer and portal vein transferred liver cancer tissues.The expression values of the above four groups are respectively 113.30 ± 11.79,137.59 ±6.23,148.78 ±8.23 and 160.28 ±9.47.Variance analysis shows that the differences of UC00lkfo expression have statistically significance ( P ＜ 0.01 ).The differences of four groups have statistically significance ( P ＜ 0.01 ),which can be arrayed as:liver cirrhosis group ＜ tissues near the cancer ＜ liver cancer group ＜ portal vein cancer embolus group.The ACTA2 values of the above four groups are respectively 109.89 ± 9.74,125.22 ± 32.16,149.06 ±8.43 and 156.57 ±8.86.Variance analysis shows that the differences of ACTA2 expression have statistically significance (P ＜0.01 ).The differences of four groups have statistically significance (P ＜ 0.05) which can be arrayed as:liver cirrhosis group ＜ tissues near the cancer ＜ liver cancer group ＜ portal vein cancer embolus group.Conclusion Expression of Uc001kfo and ACTA2 are obviously increased in liver cancer,especially the portal vein liver cancer embolus group.UC001kfo may have participated in the occurrence of liver cancer by regulating and controlling the expression of ACTA2 and is related to the invasion and metastasis of liver cancer. Key words: Hepatocellular carcinoma;  Long non-coding RNA;  UC001kfo;  Tumor metastasis