Method for detecting egg laying amount of insects by heat shock protein 83-1 gene
2013
The invention discloses a method for detecting the egg laying amount of insects by a heat shock protein 83-1 gene, which provides application of a substance which is used for detecting the expression quantity of heat shock protein 83-1 encoding genes of insect populations eating different substances to detection of the egg laying amount of insect populations eating different substances, wherein the amino acid sequence of heat shock protein 83-1 is a sequence 2 in the sequence table. Experiments prove that the detection method provided by the invention can be used for detecting the egg laying amount of the insects and particularly the egg laying amount of arma chinensis with different nutrition sources, and the egg laying amount of the insects can be detected within only 2 to 3 days by the detection method while the detection requires 30 to 60 days by a traditional biological method. The detection method provided by the invention has the advantages that materials have wide sources and are easily available, and the operation is simple as well as time-saving and labor-saving, thus being suitable for popularization and application in insect commodity inspection and detection.
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