Development and evaluation of Staphylococcus aureus-induced mastitis model.

Mastitis models were developed by inoculating mammary gland of lactating mice with different doses of Staphylococcus aureus isolate 834 through the route of injecting directly into the base of mammary gland or into the teat canal after cutting the very near end of teats.The clinical signs,histopathology,and bacterial growth in the mammary gland of the two models were compared.The results indicated that injecting into the teat canal after cutting the very near end of teats induced more typical mammary infection for the higher number of bacteria in mammary,more distinctive histopathology and better reproducible.This model could be useful for research of pathogenesis,treatment method and vaccine evaluation of Staphylococcus aureus-induced mastitis.