First Isolation and Identification of SARS-CoV-2 in Guangdong Province, China/ 广东省首例新型冠状病毒的分离与鉴定

In January 2020, Guangdong Province, China has imported several suspected cases with SARS-CoV-2 from Wuhan City, Hubei Province, China, and has been detected as SARS-CoV-2 positive in laboratory To further understand the SARS-CoV-2 virulence, as well as drug development and epidemic prevention and control needs, we established a SARS-CoV-2 isolation method Vero-E6 cells were infected with the positive bronchoalveolar-lavage sample The cells were monitored daily for cytopathic effects using light microscopy The presence of viral nucleic acid in the supernatant was detected by RT -PCR RNA extracted from culture supernatants were used as a template to clone and sequence the genome We used Illumina sequencing to characterize the virus genome and the result showed that the isolated virus was SARS-CoV-2