Nitric oxide induces apoptosis by activating K+ channels in pulmonary vascular smooth muscle cells

Nitric oxide (NO) is an endogenous endothelium-derived relaxing factor that regulates vascular smooth muscle cell proliferation and apoptosis. This study investigated underlying mechanisms involved in NO-induced apoptosis in human and rat pulmonary artery smooth muscle cells (PASMC). Exposure of PASMC to NO, which was derived from the NO donorS-nitroso-N-acetyl-penicillamine, increased the percentage of cells undergoing apoptosis. Increasing extracellular K+ concentration to 40 mM or blocking K+ channels with 1 mM tetraethylammonia (TEA), 100 nM iberiotoxin (IBTX), and 5 mM 4-aminopyridine (4-AP) significantly inhibited the NO-induced apoptosis. In single PASMC, NO reversibly increased K+ currents through the large-conductance Ca2+-activated K+(KCa) channels, whereas TEA and IBTX markedly decreased the KCa currents. In the presence of TEA, NO also increased K+ currents through voltage-gated K+(Kv) channels, whereas 4-AP significantly decreased the Kv currents. Opening of KCa channels with 0.3 mM dehydroep...