Pharmacological Effects Of Trigonella Foenum Grecum L. Seeds On Cardiovascular And Antioxidant Stress Related Disease

In this present study we mainly focus to find out the antioxidant activity of Trigonella foenum grecumL seeds. In order to provide valuable documentation that supports and strengthens the antioxidant efficacy, we attempt to compare the clinical significance of the bioactive compounds already identified by GC-MS from these seeds. DPPH, SOD and CAT assays were used as standard methods to determine antioxidant activity. The concentration of the sample required to scavenge 50% of free radical, was calculated by plotting a graph between % inhibition vs concentration. The IC50 value of DPPH radical activity for aqueous and methanolic extract was found to be 3.8741 and 4.8372.The value is almost in the range of control ascorbic acid 3.1391.The % inhibition and specific activity ofCAT for aqueous extract was found to be 78.38% and 35.227μmolesof H2O2/min/ml.The% inhibition and specific activity ofCAT for methanolic extract was found to be 83.42% and 27.0153μmoles of H2O2/min/ml. The % inhibition and specific activity of SOD for aqueous extract was found to be 54.21% and 1.0843Units/mg. The % inhibition and specific activity of SOD for methanolic extract was found to be 60.24% and1.2848Units/mg. This result suggests that the methanolic extract from the seeds of Trigonella foenum grecum L had a more powerful antioxidant activity than the aqueous extract. The result of this in-vitro study confirmed that this species of Trigonella foenum grecum L authenticated to uses as raw drug, and definitely acts as best remedy to treat cardiovascular disease. In the future, in-vivo experiments may be performed to determine the antioxidant potential of these seeds of this species in the living system.