Establishment and application of real-time PCR for quantitative detection of Salmonella spp

To establish real-time PCR for quantitative detection of Salmonella spp.,a pair of primers and fluorescent probe were designed according to stn gene sequence of Salmonella spp.,and then a real-time PCR method was successfully established to detect Salmonella spp.through the exploration of reaction system and reaction conditions.Results demonstrated that the method is specific and sensitive.Salmonella spp.detected by this real-time PCR was positive while non-Salmonella one was negative.The correlation coefficient of standard curve(R2)was 0.993 and the detecting threshold was up to 5 CFU.After the detection of 108 chicken feces,50 chicken meats and 58 water samples were detected with the this method and the positive rates were 3.7%(6/108),4%(2/50)and 3.4%(2/58)respectively.The result was consistent with results from traditional microbiology method.The method is simple,rapid,specific and sensitive,and this study made a good foundation for rapid detection of Salmonella spp.in the environment study or disease diagnosis.