Study on extraction technology of berberine by enzymatic hydrolysis

Enzymatic hydrolysis extraction of berberine was optimized by an orthogonal experiment.The effects of enzymatic hydrolysis temperature,the time of enzymatic hydrolysis,pH value of the enzymatic solution and the amount of enzymatic on the extraction yield of berberine were investigated.The optimum extraction technology of berberine was determined as follows:enzymatic hydrolysis temperature is 40℃,enzymatic hydrolysis time is 80 min,pH value of the enzymatic solution is 4.0,the amount of enzymatic is 30 mg/g.Under the optimized condition,compared traditional extraction technology with alcohol,the extraction yield of berberine was increased by 49%.