Study on the effects of bisphenol A on the morphology and β-HCG secretion of human early villus.

[Objective] To study the effects of bisphenol A(BPA)on the morphology and β-HCG secretion of human early villus and the posible toxicity of BPA to human early villus[. Methods] The human early villus from forty preganancy cases(6-8 weeks)were collected,cultured and treated with BPA at different concentrations. The cultures were divided into 4 groups based on the concentration of BPA. The concentration of BPA was 1 000×10-6 g/L in group A,500×10-6 g/L in group B,1×10-6 g/L in group C respectively,and without BPA in group D as control. The concentration of β-HCG in the supernatant of culture was measured by chemiluminescence immunoassay at day 2 and day 4 of culture,and the cultured villus was stained by HE at day 4.[Results] ① The concentration of β-HCG in the culture was elevated in A,B and C group with increasing culture time,while its concentration in D group was decreased. The concentration of β-HCG was statistically higher in group A,B and C than in group D. The concentration of β-HCG was increased with the increasing BPA.The differences of β-HCG values between day 4 and day 2 among the groups were statistically differen(tP = 0.002). ② The histologic abnormalities of villus were characterized by increasing syncytiotrophoblast knotting,prominent cytotrophoblast proliferating,basement membrane thickening,fibrinoid necrosis and anaplasia. In addition,atrophy was also observed in some villus.[Conclusion] In culture,BPA is cytotoxic to villus and has effects on the secretion of trophocyte.