Comparative immunohistochemical study of C1G5F2 antigen and myelin/oligodendrocyte glycoprotein (MOG) expression in brain of several animal species.

Abstract The localisation of the myelin/oligodendrocyte specific antigen C1G5F2 and the myelin/oligodendrocyte glycoprotein (MOG) was studied in parallel in the brain of various species throughout the phylogenetic line of vertebrates. This immunofluorescence study was performed on unfixed brain sections by using the newly described monoclonal antibody C1G5F2 and polyclonal anti-MOG antibody. The antigen C1G5F2 is detected from the reptilian class onwards whereas MOG is only found in mammals. Both antibodies clearly stained only the myelin sheaths in the brain of adult animals. The phylogenetic distribution of the C1G5F2 antigen compared to the other well known myelin proteins may indicate that it has a specific function during myelination. Moreover, evidence is given that the C1G5F2 antigen is a new minor myelin protein distinct from the glycoprotein MOG.