倉鼠頰囊袋鱗狀上皮細胞癌GST-P mRNA表現之研究

Placental glutathione S-transferase (GST-P) has been regarded as a tumor marker in many types of cancers including oral squamous cell carcinoma. The objective of this study is to investigate the expression of GST-P mRNA in DMBA-induced squamous cell carcinoma of hamster buccal pouch mucosa. Ten Syrian golden hamsters in the experimental group were painted with 0.5% DMBA in the buccal pouches for 12 weeks (three times a week). Other ten hamsters in the control group were similarly treated with mineral oil. After 12-week painting, all the animals were sacrificed and their pouches were removed. Half of these pouches were histologically examined. Other half of the pouches were used for total RNA extraction and RT-PCR was then performed to analyze the GST-P mRNA expression. Grossly and histologically, all the pouches of the DMBA-treated group showed well- differentiated squamous cell carcinoma, however, those of the control group remained unchanged. The results of RT-PCR revealed overexpression of GST-P mRNA in the buccal pouches of the experimental group, while no expression in control group was noted. These results were consistent with our previous works using immunohistochemical technique. However, factors involving overexpression of mRNA are vast and the mechanism underlying the overexpression of GST-P mRNA needs further elucidation.