Studies on chemical analysis of mycotoxin (XXI). A rapid analytical method for aflatoxins by immunoaffinity column chromatography and high performance liquid chromatography

: An analytical method for aflatoxins using solvent extraction, affinity column (containing monoclonal antibodies against aflatoxins bound onto agarose) clean-up and high performance liquid chromatographic determination was applied to the analysis of aflatoxins in peanuts, pistachio nuts, white and black peppers and corn. Recoveries of aflatoxins B1, B2, G1 and G2 spiked to peanuts, pistachio nuts and corn at the level of 20 ppb were 77.4-81.4, 70.1-77.9 and 72.3-94.8%, respectively. In the case of white and black peppers, because the spiked aflatoxin recovery rate was low, further purification steps should be studied. The stability of the affinity column was good. This procedure is recommended as a rapid and reliable method for the analysis of aflatoxins in the three kinds of foods, peanuts, pistachio nuts and corn.