Expression of class I and class II anti-HLA antibodies after implantation of cryopreserved valved allograft

Objective:To explore the humoral immunological response induced by classⅠand class Ⅱ anti-human leucocyte antigen(HLA)antibodies after implantation of conduit valved homograft(CVH).Methods:From October 2001 to October 2003,20 patients suffered from complex congenital heart disease(CHD)underwent transplantations of cryopreserved homografts artery.The other 20 complex CHDs underwent corrected operation without CVH implantations.The serums were taken and the frequency of panel reactive antibodies directed against HLA classⅠ(HLA-A,B,and C)and class Ⅱ(HLA-DR/DQ)alloantigens were detected by LAT ELISA kit before and 1 month,3 months,1year after operation.Results:The antibodies were both negative before and after operation in control patients.In CVH implanted patients,the frequency of panel reactive antibodies against HLA classⅠwas(9.28±6.64)% at 1 month,(62.14±11.95)% at 3 months and(66.79±13.42)% at 1 year after CVH implantation;that against HLA classⅡ was(22.92±15.74)% at 1 month,(41.67±18.73)% at 3 months and(52.92±20.01)% at 1 year after operation.And the panel reactive frequency of classⅠand class Ⅱ anti-HLA antibodies presented an upward trend within 1 year and a negative correlation with age.Age negatively correlated with the degree of elevation of classⅠand class Ⅱ anti-HLA antibodies.Conclusion:Implantation of CVH can induce a marked humoral immunological response involved classⅠand class Ⅱ anti-HLA antibodies;this response is stronger in young than in eld.