The significance of the vascularization of embryonic donor brain for the viability of intraocular transplants in the rat

: Telencephalic regions of rat embryonic brain were transplanted into the adult rat eye anterior chamber on E12 and E14 stages of development. These stages fall on the onset of the vascularization and the presence of the well developed vascular network in the embryonic brain. After a month of cultivation E14 transplants were much more viable than E12 (appropriately 58% and 15%). Only few E12 grafts had no initial vascularization. These transplants displayed growth retardation, while differentiated neurons could be seen only on their periphery. The results of the investigation demonstrate the meaning of vascularization extent in donor embryonic nervous tissue in its survival in conditions of transplantation into the rat eye anterior chamber.