A facile one-step synthesis of 5′-phosphatidylnucleosides by an enzymatic two-phase reaction.

Abstract Phospholipase D from Streptomyces effectively catalyzed the transfer reaction of the phosphatidyl residue from phosphatidylcholines to the 5′-hydroxyl group of nucleosides in a two-phase system. A variety of 5′-phosphatidylnucleosides were easily prepared in high yields by this reaction.