Successful hybridization of groupers (Epinephelus coioides x Epinephelus lanceolatus) using cryopreserved sperm

Abstract This study examined the effect of three extenders, Marine Fish Ringer (MFR), sodium citrate (CT), and sodium chloride (NaCl) and two cryoprotectants (10% dimethyl sulfoxide—DMSO and 15% Trehalose) on the cryopreservation of giant grouper, Epinephelus lanceolatus sperm which was later used for hybridization trial with orange-spotted grouper, Epinephelus coioides . Fish Ringer and sodium citrate as extenders resulted in higher fertilization (71 ± 3 and 68 ± 4%, respectively) than NaCl (58 ± 1%). There was no difference in percent fertilization and hatch between DMSO and Trehalose and all combinations of extenders and cryoprotectants tested. Successful hybridization of E. coioides x E. lanceolatus was achieved for the first time between these two species using cryopreserved sperm from giant grouper. There was no difference in percent fertilization and hatch between hybrid and nonhybrid, orange-spotted grouper, but percent deformity of the hybrids (47%) was higher ( P E. coioides x E. lanceolatus hybrid offsprings thus, laying the groundwork for further comparative studies on hybrid performance.