immunogenic composition or vaccine against gram-negative bacterial disease or infection, for example, Neisseria

A vaccine for use in the treatment or prevention of an infection or disease by Gram negative bacteria comprising: (I) a first antigen that is capable of eliciting an immune response, if necessary when coupled to a carrier protein, the immune response It recognizes an outer membrane protein of at least a first Gram-negative bacterial strain, wherein said outer membrane protein is involved in the uptake of extracellular zinc, wherein said first antigen comprising: a. a polypeptide having at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95 %, at least 99% or at least 100% identity with the amino acid sequence of SEQ ID No. 1, or b. a polypeptide comprising an immunogenic fragment of the sequence of SEQ ID No. 1, wherein said immunogenic fragment consists of a sequence of amino acids having 7, 8, 9, 10, 11, 12, 13, 14, 15, 20 , 30, 40, 50 or more contiguous amino acids of SEQ ID No. 1, and (II) a second antigen, wherein said second antigen comprises: a. a polypeptide having at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95 %, at least 99% or at least 100% identity with the amino acid sequence of SEQ ID No. 50; optionally wherein said second antigen is capable of eliciting an immune response, if necessary when coupled to a carrier protein, the immune response recognizes the amino acid sequence of SEQ ID NO. 50, or b. a polypeptide comprising an immunogenic fragment of the sequence SEQ ID No. 50, wherein said immunogenic fragment consists of a sequence of amino acids having 7, 8, 9, 10, 11, 12, 13, 14, 15, 20 , 30, 40, 50 or more contiguous amino acids of the sequence SEQ ID No. 50, and wherein said immunogenic fragment is capable of eliciting an immune response, if necessary when coupled to a carrier protein, the immune response recognizes the amino acid sequence of SEQ ID NO. 50, wherein said vaccine comprises a vesicle preparation bacterial outer membrane comprising said first antigen, optionally wherein expression of said first antigen upregulated in said preparation of outer membrane vesicle.