Protein and Peptide Sequence Analysis by Tandem Mass Spectrometry in Combination with Either Capillary Electrophoresis or Micro-Capillary HPLC

Sequence analysis of peptides in mixtures by the combination of capillary electrophoresis, electrospray mass spectrometry is described. On-column digestion of proteins at the 1–10 pmol level followed by on-line separation and sequence analysis of the resulting peptides by micro-capillary HPLC/electrospray mass spectrometry is also presented.