Nonviral gene editing in cancer immunotherapy

Abstract This chapter discusses the design principles and applications of nonviral delivery systems for clustered regularly interspaced short palindromic repeat/Cas9-based genome editing platforms for potential cancer immunotherapy. It starts by briefly introducing the gene editing technologies and intracellular delivery strategies. It then explains the design principles of representative studies that used nonviral fully-synthetic inorganic and organic nanomaterials for immune-cell-targeted delivery of bioactive components and discusses their potential applications to cancer immunotherapy. Overall, the field of nonviral genome editing systems in cancer immunotherapy is nascent. This area is thus a forefront of biomedical research and deserves further exploration in the future. This chapter also discusses the challenges and opportunities of the development of nonviral genome editing systems in cell-based cancer immunotherapy from a materials science perspective.