Fabrication of glycosylated surface on polymer membrane by UV-induced graft polymerization for lectin recognition.

Increasingly, carbohydrate−protein interactions are viewed as important mechanisms for many biological processes such as blood coagulation, immune response, viral infection, inflammation, embryogenesis, and cellular signal transfer. However, the weak affinity of the interactions and the structural complexity of carbohydrates have hindered efforts to develop a comprehensive understanding of carbohydrate functions. Fortunately, synthetic polyvalent glycoligands give us a chance to reveal the nature of these biological processes. In this work a sugar-containing monomer (α-d-allyl glucoside (AG)) was grafted onto polypropylene microporous membrane (PPMM) by UV-induced graft polymerization to generate a glycosylated porous surface for the first time. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy were employed to confirm the glycosylation. Water contact angle measurement was used to evaluate the hydrophilicity change of the surfaces before and after ...