Polyclonal antibody and immunomagnetic beads preparation technology for type A trichothecenes

OBJECTIVE: Preparing immunomagnetic beads for enrichment and purification of mask type A trichothecenes. METHODS: Through immunizing New Zealand white rabbits with family A toxin artificial antigen 3-Ac-NEOS-HS-BSA, the polyclonal antibodies of type A trichothecenes( type A family) were isolated, as well as its antibody titre were determinated and purified. The coupling condition of high titer antibody and immunomagnetic beads were optimized and applied. RESULTS: After 5 times of immunization with type A trichothecene family toxin, the rabbit serum inhibited the highest antibody titer( 1 : 64 000). The optimal coupling condition of antibody and immunomagnetic beads were: temperature = 24 ℃, rotation capture in pH 7. 4, 0. 02 mol/L PBS buffer solution with 10% methanol in 24 h. CONCLUSION: The establishment of preparation method of type A single family toxin polyclonal antibody and immunomagnetic beads can make a standard for enrichment and purification of type A matrix complex single family toxins.