Additional file 1: Figure S1. of Integrin-β5, a miR-185-targeted gene, promotes hepatocellular carcinoma tumorigenesis by regulating β-catenin stability

MHCC-97 L cell lysates were incubated with IgG (control) or ITGB5 antibody. The bounded proteins were eluted, resolved by SDS-PAGE, and visualized by CBB staining (upper panel). The band in the ITGB5 lane was identified as β-catenin by mass spectrometry. The obtained peptide sequences by mass spectrometry analysis were shown on the right.(B-D)The RNA levels of miR-185 were analysed by q-RT-PCR. (TIFF 552 kb)