Cross-reactivity between pollen extracts from six artemisia species.

Pollen extracts of six different Artemisia species, A. vulgaris, A. scoparia, A. princeps, A. tridentata, A. annua, and A. campestris were compared using SDS-PAGE, IEF, immunoblotting, and immunoelectrophoretic methods. The band patterns obtained after SDS-PAGE and IEF showed a large degree of similarity between the extracts. Immunoblotting of these gels using a pool of sera from patients allergic to A. vulgaris gave essentially the same IgE-binding band pattern with all the extracts, demonstrating an extensive degree of crossreactivity between A. vulgaris and the other Artemisia species