Apparatus for rapidly and semi-quantitatively detecting uric acid in saliva, and making method thereof

The invention relates to an apparatus for rapidly and semi-quantitatively detecting uric acid in saliva, and a making method thereof, and belongs to the field of medical detection apparatuses. The apparatus is obtained through pasting a nitrocellulose membrane of solid phase uric acid-carrier protein and goat anti-rabbit IgG polyclonal antibody, glass fibers adsorbed with colloidal gold labeled anti-uric acid antibody, a sample pad, absorbent paper and other auxiliary materials. The apparatus has the advantages of novel structure, strong specificity, rapid and semi-quantitative detection of the content of uric acid in saliva, and no increase of the production operation complexity. The nitrocellulose membrane is preprocessed with a polylysine treatment liquid, the uric acid-carrier protein and silica nanoparticles are combined, the obtained combination material is adsorbed to the nitrocellulose membrane, and a proper gold spray buffer solution and a proper sample pad treatment liquid are matched, so the reaction sensitivity is effectively improved on the basis of guaranteeing of complete release of immune colloid gold, the use amount of the immune colloid gold is reduced under same threshold, and the cost is saved. The detection apparatus has the advantages of high sensitivity, strong specificity, simple operation, time saving and strong practicality.