Prokaryotic expression of coat protein of Cherry virus A isolate Beijing and preparation of the polyclonal antiserum.

According to previous work,Cherry virus A Beijing isolate(CVA-BJ) cp gene was cloned into pET-28a vector and fusion protein was expressed in Escherichia coli strain BL21(DE3).After purification through affinity Ni column,the fusion protein was used to immunize the white rabbit to produce antiserum.The titer of the antiserum was 1∶2 048 tested by indirect ELISA.Western blot analysis indicated that the antiserum was specific to CVA.With diseased cherry leaves confirmed by RT-PCR as test material,positive reactions occurred in indirect ELISA test.The results indicated that the antiserum could be applied in detecting CVA in the field.