Transferrin gene cloning and sequencing analysis from Indonesian Nile Tilapia (Oreochromis niloticus)

Some fish have ability to live in the wide salinity range. Nile Tilapia (Oreochromis niloticus) is one example of fish that can tolerate the changes of the salinity in the water. Transferrin is one of many factors that makes Nile Tilapia fish can live in very wide salinity range. In Indonesia, Nile Tilapia usually lived in freshwater area. The purpose of this research is to clone and sequencing transferrin gene in Indonesian Nile Tilapia fish (Oreochromis niloticus). The subject of this research are three variants of Nile Tilapia fish which represent the population of Nile Tilapia fish in Indonesia, which is GESIT, Red Nifi, and Selfam. The DNA was taken from caudal fin and extracted by Phenol Chloroform Isoamyl-alcohol (PCI) method. Transferrin gene was amplified using TRF F2 primer (TTGACAACTATRAMRCCTGC TYCC) and TRF R1 primer (CAGCSGTAAACACCTGACCT) which resulted 800 bp DNA fragment. The DNA fragment from the agarose gel is then cut and purified. Transferrin gene fragment was cloned using pGEM®-T easy ...