A New Method for Renal Perfusion Fixation

Kidneys can tolerate ischemia lasting up to 2 h at a temperature of about 30 °C when protected by HTK (histidine trytophane ketoglutarate) (Groenewoud et al. 1989; Gubernatis et al. 1990; Isemer et al. 1988; Kallerhoff 1989; Kehrer et al. 1989; Pichlmayr et al. 1990; Preusse et al. 1987; Schnabel et al. 1987). Structural recovery after such ischemic stress as examined by light microscopy on immersion-fixed sections — is completed after 2–3 h reperfusion. There are no visible structural differences to normal kidney tissue. This was the reason for also testing this solution for improvement of renal perfusion fixation.