Effect of celecoxib on pulmonary hypertension of chronic hypoxia and hypercapnic rats

Objective: To study the effect of celecoxib on chronic hypoxia and hypercapnic pulmonary hypertension.Methods: SD rats were randomly divided into normal control group(A),hypoxic hypercapnic group(B),hypoxic hypercapnia+ celecoxib group(C).The content of TXB2 and 6-keto-PGF1α in plasma and lung were detected by the technique of radioimmunology.Results: ①Mean pulmonary arteria pressure(mPAP) was significantly higher in rats of B group than those of A group.mPAP was significantly higher in rats of C group than those of B group.Differences of mPAP were not significant in three groups.②The content of TXB2 in plasma and lung and the ratio of TXB2/6-keto-PGF1α were significantly higher in rats of B group than those of A group.The ratio of TXB2/6-keto-PGF1α was significantly higher and the content of 6keto-PGF1α in plasma and lung was significantly lower in rats of C group than those of B group.③Light microscopy showed that WA/TA(vessel wall area/total area) and PAMT(the thickness of medial smooth cell layer) were significantly higher in rats of B group than those of A group.WA/TA and PAMT were significantly higher in rats of C group than those of B group.④Electron microscopy showed the thickening of vessel wall and the proliferation of collagen fiber in B group and augmentation of smooth muscle cell and abundance of myofilament in pulmonary arterioles in C group.Conclusion: Celecoxib can aggravate hypoxic hypercapnia pulmonary hypertension and pulmonary vessel remodeling by increasing the ratio of TXA2/ PGI2.