Head-to-Head Comparison of Inflammation and Neovascularization in Human Carotid PlaquesCLINICAL PERSPECTIVE: Implications for the Imaging of Vulnerable Plaques

Background— Inflammation and intraplaque neovascularization are acknowledged to be 2 features of plaque vulnerability, although their temporal expression and their respective value in predicting clinical events are poorly understood. To determine their respective temporal associations, we conducted a comprehensive assessment of inflammation and intraplaque neovascularization in the carotid plaque of symptomatic and asymptomatic patients. Methods and Results— Thirty patients with severe carotid stenosis underwent 18 F-fluorodeoxyglucose-positron emission tomography/computed tomographic imaging. Plaque 18 F-fluorodeoxyglucose-uptake, indicative of inflammation, was measured by calculating the target:background ratio. The presence of intraplaque neovascularization during contrast-enhanced ultrasound was judged semiquantitatively; low-grade contrast enhancement (CE) suggested its absence, and high-grade CE, the presence of neovascularization. Carotid surgery was performed 1.6±1.8 days after completing both imaging modalities in all patients, and the presence of macrophages and neovessels was quantified by immunohistochemistry. We identified a significant correlation between the target:background ratio and macrophage quantification ( R =0.78; P P P =0.28 and P =0.60, respectively) nor macrophage infiltration ( P =0.59 and P =0.40, respectively). Finally, macrophage infiltration and target:background ratio were higher in the carotid plaque of symptomatic patients ( P =0.021 and P =0.05, respectively), whereas CE grade and the presence of neovessels were not. Conclusions— Inflammation and intraplaque neovascularization are not systematically associated in carotid plaques, suggesting a temporal separation between the 2 processes. Inflammation seems more pronounced when symptoms are present. These data highlight the challenges that face any imaging strategy designed to assess plaque vulnerability.