Bovine viral diarrhea virus (BVDV) transgenosis astragalus mongholicus vaccine and production method

The invention relates to a bovine viral diarrhea virus (BVDV) transgenosis Astragalus mongholicus vaccine and a production method which are used for preventing and curing animal bovine viral diarrhea virus, and overcoming the defects of poor immunogenicity and low protection of the existing inactivated vaccines and nucleic acid vaccines as well as unsafe application of the existing live poison vaccine. In the invention, BVDV RNA is taken as a template, a reverse transcription-polymerase chain reaction method is used for obtaining an E0 gene thereof, then a gene E0 recombination plant expression vector is constructed, a pollen tube pathway method is used for obtaining the BVDV E0 gene transgenosis Astragalus mongholicus, and the Astragalus mongholicus with immunogen transformed by the BVDVE0 gene is screened by an immunoblotting and an enzyme linked immunosorbent assay to obtain a vaccine product. The invention has the following beneficial effects: the BVDV E0 gene is expressed in theAstragalus mongholicus, which generates the protective antigen and adjuvant substance capable of increasing the immunization, thereby improving the antiviral effect of the Astragalus mongholicus and the optimun immunity of the vaccine; and the invnetion has the advangtages of easy production, convenient use, strong stability and no side effects.