Plasma level of antioxidants is related to frequency of vaso-occlusive crises in children with sickle cell anaemia in steady state in nigeria.

Abstract Introduction Sickle cell anaemia (SCA) is associated with deficiency of plasma antioxidants. Frequency of vaso-occlusive crises (VOC) is an index of disease severity. This study determined the relationship between plasma antioxidants in children with SCA and frequency of VOC. Method ology : It was a cross-sectional comparative study comprising 80 children each with SCA and HbAA. Those with SCA were partitioned into three groups based on frequency of pain episodes. Antioxidant trace elements (zinc, copper, selenium, and manganese), vitamins A, C and E and antioxidant enzymes (superoxide dismutase and glutathione dismutase) were quantified using atomic absorption spectrophotometry, HPLC and ELISA respectively. Results Children with SCA had lower plasma concentrations of zinc, selenium, manganese, vitamins A, C, E, and superoxide dismutase (SOD) and glutathione peroxidase (GPx), but higher plasma concentration of copper compared to HbAA. Mean plasma concentrations of manganese, vitamin A, vitamin C, vitamin E, SOD and GPx were significantly lower in SCA children with severe disease compared to those with mild and moderate disease (F=10.629, p Conclusion Deficiency of plasma antioxidants exists in SCA and plasma levels of some antioxidants have inverse relationship to frequency of VOC.