Antibody subclass skewing predicts enhanced ADCC activity in both natural infection and vaccination

Background The innate immune recruiting property of antibodies are elicited following an Fc/Fc-receptor interaction. We previously demonstrated that HIV-specific antibodies from elite controllers (ECs) robustly recruit NK cells to mediate antibody dependent cellular viral inhibition (ADCVI) compared to antibodies from chronic progressors. To gain further insights into the biophysical properties of EC antibodies that enable them to recruit Fc-effector functions so robustly, we performed at 24 dimensional analysis (including specificity, isotype, Fc-receptor affinity, and function) of the antibody profiles mounted in ECs and HIV-progressors to define the humoral signature(s) associated with most robust innate immune recruiting activity.