Green Synthesis of Silver Nanoparticles from Ananas comosus Core Extract and Their Antibacterial Activity

Some research has been made for the green synthesis of silver nanoparticles from plant extracts however it is still limited. Therefore, this study was conducted to study the presence of green silver nanoparticles and antibacterial activity from Ananas comosus core extract. The core was extracted with sterile distilled water and centrifuge at 6000 rpm before incubated at 70 ℃ for 12 h. Bioreduction of Ag+ to Ag0 was identified through visual observation from the changes of resultant extract colour from colourless to dark brown. The formation of nanoparticles was identified at 24 and 72 h using UV-vis spectroscopy. The antibacterial activity of AgNPs was investigated at concentration ratios of 1:2 and 1:4 against Escherichia coli and Staphylococcus aureus. From the result, the spectrum showed a peak between 421 and 424 nm corresponding to the plasmon absorbance of the AgNPs at 72 h of reaction time for both ratios. The results also indicated that at concentration ratio of 1:2 and 1:4 able to inhibit bacterial growth for E. coli and S. aureus with the highest inhibition zone at 17.67 ± 1.15 mm and 11.67 ± 0.57 mm for E. coli while S. aureus at 14.0 ± 1.0 mm and 13.0 ± 1.0 mm, respectively. The green approach demonstrated the potential to synthesis silver nanoparticles using plant extract and have capability to inhibit the bacterial.