Peptide Analysis Using Tandem Mass Spectrometry

Abstract : The objective of the project was to determine the complete amino acid sequence of the large polypeptide Ubiquitin by use of fast atom bombardment (FAB ionization and tandem mass spectrometry. The peptide containing 76 amino acid residues was available at Warwick University as 12 tryptic digests which had previously been separated by off-line reverse phase HPLC. Most of the peptides resulting from tryptic digest contained four ten amino acid residues and were of molecular weights below 2,000 daltons. Keywords: Amino acid sequences.